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KMID : 0371919890020010149
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Journal of Wonju College of Medicine
1989 Volume.2 No. 1 p.149 ~ p.159
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A Study of Toxins of Korean Fatally Poisonous Mushroom
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Abstract
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The mushrooms of the genus Amanita some of that are poisonous to man, occasionally even deadly, are widely spread through the world.
In Korea, the deaths from the mushroom poisonings were not uncommon and guessed to be due to Amanita phalloides from the clinical pictures of cases of fatal mushroom poisonings, ie clincal pictures of liver failure. but it came true that in fact, deaths from such fatal mushrooms poisonings were due to Amanita virosa identified by morphology and 3% KOH dropping test and according to Department of mycology, Institute of Agricultural Sciences, Rural Development Administration the Amanita virosa were common and moreover the Amanita phalloides, so far, were not discovered in Korea.
Amatoxin and phallotoxin, a low molecular weight, thermo-and alcohol-stable toxins were detected by Biogel P-2 gel filtration chromatography; 0.5㎖ of eluate was lethal to mice 2-4hrs after i.p. injection.
Short-time heating to 100℃ did not detoxify the eluate. Hemolytic activity containing molecule, a high molecule weight, thermo-and alcohol-labile toxin was demonstrated in Amanita virosa. Hemolytic activity containing molecule has been purified 16:9 folds from Amanita virosa by a series of steps including cold aqueous extracts by with 40% ammonium sulphate, DEAE-sephecel ion exchange chromatography, Biogel P-30 gel filtration chromatography and electrophoretic elution.
After electrophoretic elution, 10 ul of eluate was lethal to mice 0.5-1 hr after i. p. injection. Short-time hearting to 100℃ detoxify the eluate.
Further experiments are in progress to chracterize the physiochemical and biochemical properties of this hemolytic containing molecule.
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KEYWORD
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